phenotyper 3000 cages  (Noldus Information Technology)

Journal: Disease Models & Mechanisms

Article Title: The behavioural consequences of dystrophinopathy

doi: 10.1242/dmm.052047

Figure Lengend Snippet: Serial reversal learning with food reward in PhenoTyper cages. Mdx 5cv ( n =9-10), mdx52 ( n =14), DMD-null ( n =10-15) and WT ( n =32-33). (A) Discrimination learning (left target). Both mdx 5cv and mdx52 mice showed steeper learning curves than those of WTs ( P< 0.001 and P= 0.011, respectively) and DMD-null mice ( P< 0.001 and P= 0.018, respectively). (B) Reversal day 1 (right target). Mdx 5cv mice showed decreased learning compared to WTs ( P =0.017). (C) Reversal day 2 (left target). Mdx52 mice showed increased performance compared to WTs and mdx 5cv mice ( P< 0.001 and P= 0.013, respectively). (D) Reversal day 3 (middle target). Mdx52 mice showed increased learning compared to WTs ( P= 0.018). (E) Reversal day 4 (right target). No differences were found between groups. (F) Reversal day 5 (left target). No differences were found between groups. All graphs were cut off at n= 3 for visual purposes. Cartoons were created in BioRender by Verhaeg, M. (2025). https://BioRender.com/e60i623 . This figure was sublicensed under CC-BY 4.0 terms.

Article Snippet: PhenoTyper automated home cages (model 3000, Noldus Information Technology) consist of transparent Perspex walls (30×30×35 cm) and opaque Perspex floors and an infra-red camera in the top unit.

Techniques:

Journal: Disease Models & Mechanisms

Article Title: The behavioural consequences of dystrophinopathy

doi: 10.1242/dmm.052047

Figure Lengend Snippet: Spontaneous behavior in the PhenoTyper cages: activity-based behavior. Mdx 5cv ( n =14), mdx52 ( n =14), DMD-null ( n =16) and WT ( n =33). (A) Mdx52 mice showed a higher habituation index during the dark phase compared to WT, mdx 5cv and DMD-null mice ( P< 0.001, P= 0.025 and P <0.001, respectively). (B) Mean activity duration during the dark phase was lower in DMD-null mice than in WT and mdx 5cv mice ( P= 0.002 and P< 0.001, respectively). (C) In DMD-null mice, activity in anticipation of the dark phase was less changed than in WT mice ( P =0.004). (D) DMD-null mice showed less change in activity in response to the start of the dark phase than WTs ( P= 0.023). (E) Mdx52 mice showed a higher increase in activity in anticipation of the start of the light phase than WTs ( P= 0.032). (F) Mdx52 mice showed a lower decrease in activity change in response to the start of the light phase than WT and mdx 5cv mice ( P= 0.037 and P< 0.001, respectively). DMD-null mice also showed less of a decrease in activity than mdx 5cv mice ( P< 0.001). * P< 0.05, ** P< 0.01, *** P <0.001 (one-way ANOVA with post-hoc Tukey).

Article Snippet: PhenoTyper automated home cages (model 3000, Noldus Information Technology) consist of transparent Perspex walls (30×30×35 cm) and opaque Perspex floors and an infra-red camera in the top unit.

Techniques: Activity Assay

Journal: Disease Models & Mechanisms

Article Title: The behavioural consequences of dystrophinopathy

doi: 10.1242/dmm.052047

Figure Lengend Snippet: Spontaneous behavior in the PhenoTyper cages: sheltering behavior, movement and arrest. Mdx 5cv ( n =14), mdx52 ( n =14), DMD-null ( n =16) and WT ( n =33). (A) The long shelter visit threshold was increased in mdx 5cv mice compared to that in WT and DMD-null mice ( P< 0.001 and P =0.007, respectively). (B) Both mdx52 and DMD-null mice showed a lower cumulative duration of shelter visits above the long shelter visit threshold compared to that in WTs ( P< 0.001 and P= 0.009, respectively). (C) The long movement threshold was lower in DMD-null mice than in WT, mdx 5v and mdx52 mice ( P< 0.001, P< 0.001 and P= 0.027, respectively). (D) DMD-null mice had a lower duration of arrests above the threshold than WT and mdx 5cv mice ( P= 0.014 and P= 0.009, respectively). * P< 0.05, ** P <0.01, *** P <0.001 (we refer the reader to for an overview of the statistical tests performed).

Article Snippet: PhenoTyper automated home cages (model 3000, Noldus Information Technology) consist of transparent Perspex walls (30×30×35 cm) and opaque Perspex floors and an infra-red camera in the top unit.

Techniques:

Journal: Genes, Brain, and Behavior

Article Title: Learning, memory and blood–brain barrier pathology in Duchenne muscular dystrophy mice lacking Dp427, or Dp427 and Dp140

doi: 10.1111/gbb.12895

Figure Lengend Snippet: Overview of the experimental setup. Mice started between 10 and 15 weeks of age and underwent testing via T‐maze, Morris water maze (MWM), PhenoTyper cages and a series of MRI scans. AN, anxiety; DL, discrimination learning; RL, reversal learning; SB, spontaneous behavior.

Article Snippet: A PhenoTyper automated home‐cage (Noldus PhenoTyper 3000, Noldus Information Technology), with transparent Perspex walls (30 × 30 × 35 cm) and opaque Perspex floor, was used to assess the mice's behavior.

Techniques:

Journal: Genes, Brain, and Behavior

Article Title: Learning, memory and blood–brain barrier pathology in Duchenne muscular dystrophy mice lacking Dp427, or Dp427 and Dp140

doi: 10.1111/gbb.12895

Figure Lengend Snippet: Discrimination‐ and reversal learning with cognition wall and anxiety in PhenoTyper cages for WT ( n = 21), mdx ( n = 23–25) and mdx 4cv ( n = 22–23) mice. 80% success rate was calculated over a time window of 30 entries. (A) Survival curve of discrimination learning trials. Mdx and mdx 4cv mice were significantly faster in reaching the 80% criterium than WTs during discrimination learning. (B) Survival curves of reversal learning trials. No differences were found between groups. (C). Grey area (phase 2) represents the time the light was on. All groups showed a significant response to the light in phase 2 compared with baseline measurements of 24 h earlier. No differences were found between groups in the anxiety response. Asterisks indicate *** p < 0.001.

Article Snippet: A PhenoTyper automated home‐cage (Noldus PhenoTyper 3000, Noldus Information Technology), with transparent Perspex walls (30 × 30 × 35 cm) and opaque Perspex floor, was used to assess the mice's behavior.

Techniques:

Journal: Genes, Brain, and Behavior

Article Title: Learning, memory and blood–brain barrier pathology in Duchenne muscular dystrophy mice lacking Dp427, or Dp427 and Dp140

doi: 10.1111/gbb.12895

Figure Lengend Snippet: Spontaneous behavior in PhenoTyper cages for WT ( n = 21), mdx ( n = 23) and mdx 4cv ( n = 22) mice. (A) Average velocity was lower in mdx mice compared with WT mice during the dark phase. (B) The duration of active behavior was shorter in both DMD models compared with WTs in the dark phase. (C) Mdx and mdx 4cv mice showed a smaller change in activity in anticipation of the start of the dark phase, compared with WT mice. (D) Long movement threshold values revealed decreases in threshold for both mdx and mdx 4cv mice compared with WTs (E) Maximum velocity of long movement segment was decreased in mdx compared with WT mice. (F) Long movement arrest threshold was significantly lower in mdx mice compared with WTs. Asterisks indicate * p < 0.05, ** p < 0.01, *** p < 0.001. Cartoons are taken from Loos et al. 2014.

Article Snippet: A PhenoTyper automated home‐cage (Noldus PhenoTyper 3000, Noldus Information Technology), with transparent Perspex walls (30 × 30 × 35 cm) and opaque Perspex floor, was used to assess the mice's behavior.

Techniques: Activity Assay